Lysis buffer - Alchetron, The Free Social Encyclopedia
Lysis buffer - Alchetron, The Free Social Encyclopedia
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SOLVED: 1. What does the lysis buffer do? (after resuspending the cells, add lysis buffer)2. What is left out of the water control for PCR? (Restriction mapping and Ligation)3. Would you expect the plasmid that was restricted by two different restriction enzymes to be recircularized by ligase? Why or why not?
SOLVED: 1. What does the lysis buffer do? (after resuspending the cells, add lysis buffer)2. What is left out of the water control for PCR? (Restriction mapping and Ligation)3. Would you expect the plasmid that was restricted by two different restriction enzymes to be recircularized by ligase? Why or why not?
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