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Video: Separating Protein with SDS-PAGE: Procedure and Applications
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SOLVED: Determination of Molecular Weight of a ProteinYou want to determine the molecular weights of unknown proteins by using SDS-PAGE. The following table shows the migration distance of each of the standard proteins (markers):Name of proteinMolecular weight (dalton)Migration distance (mm)E. coli β-galactosidaseRabbit phosphorylase BLea albuminChicken ovalbuminChicken egg lysozyme116,00097,40066,50042,70014,300The migration distances of two unknown proteins were as follows: Unknown Protein A:
SOLVED: Determination of Molecular Weight of a ProteinYou …
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