Explain the transformation of inactive B cells into plasma cel... | Filo
Explain the transformation of inactive B cells into plasma cel... | Filo
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Simplified Cell-Free Protein Synthesis | JoVE Journal
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Brianna Bibel on Instagram: "Cloning vs. expression cells (with regards to recombinant protein production) – Rather than one or the other, it's typically one (cloning cells), then the other (expression cells)! 1. Make or obtain your plasmid 2. Transform into cloning cells to make lots of copies 3. Purify out the copies (e.g. with a miniprep) 4. Check the sequence. 5. Transform into expression cells 6. Express protein https://youtu.be/r1uwdNMTTFY cloning cells: https://bit.ly/dh5alpha expression
19:59
Brianna Bibel on Instagram: "Cloning vs. expression cells (with regards to recombinant protein production) – Rather than one or the other, it's typically one (cloning cells), then the other (expression cells)! 1. Make or obtain your plasmid 2. Transform into cloning cells to make lots of copies 3. Purify out the copies (e.g. with a miniprep) 4. Check the sequence. 5. Transform into expression cells 6. Express protein https://youtu.be/r1uwdNMTTFY cloning cells: https://bit.ly/dh5alpha expression
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Instagramthebumblingbiochemist
Brianna Bibel on Instagram: "Does the plasmid you ordered have 2 antibiotics listed? One might actually be for cells that plasmid is in (e.g. BL21(DE3)-RIL) which themselves have a plasmid, containing genes for tRNAs for rare codons… https://youtu.be/uUFuyu0gQ9k BL21 is the workhorse E. coli strain for recombinant protein expression. Extra things can be added and/or the bacterial genome can be modified to help optimize the cells for making various proteins. For example, you can add in extra copi
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Brianna Bibel on Instagram: "Does the plasmid you ordered have 2 antibiotics listed? One might actually be for cells that plasmid is in (e.g. BL21(DE3)-RIL) which themselves have a plasmid, containing genes for tRNAs for rare codons… https://youtu.be/uUFuyu0gQ9k BL21 is the workhorse E. coli strain for recombinant protein expression. Extra things can be added and/or the bacterial genome can be modified to help optimize the cells for making various proteins. For example, you can add in extra copi
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Instagramthebumblingbiochemist
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