eLife14d
Chromatin activity of IκBα mediates the exit from naïve pluripotency
This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to ...
Frontiers2y
The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation
Uncoated beads were used as a negative control ... fixed in 4% PFA for 10 min at RT and then permeabilized and blocked with 0.3% Triton X-100, 5% donkey serum in PBS, 30 min at RT. To visualize the ...
Frontiers4y
Loss of MeCP2 Function Across Several Neuronal Populations Impairs Breathing Response to Acute Hypoxia
Sections were blocked for 1 h in a PBS solution containing 10% serum (matched to the host used for the secondary antibodies) and 0.3% Triton X-100. Neuronal activation was assayed by immune-staining ...