The sections were incubated with blocking solution (10% normal goat serum (Vector Laboratories) and 0.3% Triton X-100 (Wako) in phosphate buffer) for 2 h at room temperature. After blocking, they were ...

And then, the slices were washed three times in 1 x PBS for 10 min each, followed by permeabilization in 1% triton X-100 solution in 1 x PBS for 40 min at room temperature. After blocking for 1 hour ...

And then, the slices were washed three times in 1 x PBS for 10 min each, followed by permeabilization in 1% triton X-100 solution in 1 x PBS for 40 min at room temperature. After blocking for 1 hour ...

Then, cells were fixed with 4% PFA for 10 min at RT and blocked/permeabilized for 20 min at RT (5% donkey serum with 0.3% Triton X-100 in PBS). After blocking, samples were stained with primary ...