The authors examine the role of Numb, a Notch inhibitor, in intestinal stem cell self-renewal in Drosophila during homeostasis and regeneration. This is an important study providing evidence of a Numb ...

Various clean energy technologies have been developed to meet the rapidly intensifying energy demand and dwindling fossil fuel reserves. However, many of these technologies are hindered by low ...

The hydrovoltaic fire sensor developed by researchers offers a cost-effective, efficient solution, addressing limitations of ...

The insoluble formazan crystals were dissolved using DMSO and the resulting colored solution was quantified by measuring ... cells were fixed with 4% paraformaldehyde and permeabilized using 0.2% ...

For maximum lysis, 20% Triton X-100 solution was added. After three hours of cocultivation, 20 µl of supernatant was transferred to another 96-well flat-bottom plate, and 200 µl of europium solution ...

After fixed in 4% PFA and repeated washing in glicine-PBS solution (100 mM, 15 min, 22°C), cells were permeabilized with Triton-X-100 (0.5 v/v%, 10 min, 22°C). After PBS washing (3x) non-specific ...

Cells were seeded on coverslips and treated as indicated. Cells were than fixed in cold 4% paraformaldehyde for 15 min and permeabilized in 0.25% Triton X-100 solution for 5 min at room temperature.

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Spindle assembly checkpoint (SAC) inhibitors ...