Low-salt wash buffer: 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) ... Filter the solution through two layers of Miracloth into a fresh 50-ml Falcon tube placed on ice.

Then, the cells were permeabilized using a 0.2% Triton X-100 solution (Servicebio Technology Co., Ltd., Wuhan, China) for 20 min, followed by blocking with 2% bovine serum albumin (Sangon Biotech Co., ...

Cell samples were first rinsed with PBS (3X), followed by fixation with a 4% paraformaldehyde solution at 4 °C for 30 min. Cell permeabilization was then completed with a 0.1% Triton X-100 solution ...

This valuable manuscript investigates the localisation of nutrient receptors in bloodstream stage trypanosomes, with implications for both nutrient uptake and immune evasion. Results after direct ...

Based on several lines of interesting data, the authors conclude that FMRP, though associated with stalled ribosomes, does not determine the position on the mRNAs at which ribosomes stall. Although ...