This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to ...

During the peer-review process the editor and reviewers write an eLife assessment that summarises the significance of the findings reported in the article (on a scale ranging from landmark to useful) ...

Triton X-100, SDS at a final concentration of 0.25%, 0.5% and 1%; and an oxidizing agent-H 2 O 2 at a final concentration of 0.5%, 1%, 1.5% and 2%. The incubated enzyme (without inhibitors, detergents ...

In re-ChIP experiments, the first ChIP (H2A.Z or H2A) was eluted with 10 mM Tris-EDTA and 20 mM DTT and diluted 20 times in dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl ...

Protein samples were separated on a 12% SDS-polyacrylamide gel electrophoresis (PAGE ... Platelets and U251-MG cells were permeabilized using 0.1% Triton X-100 in PBS for 10 min at RT. Coverslips were ...