This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to ...

Triton X-100, SDS at a final concentration of 0.25%, 0.5% and 1%; and an oxidizing agent-H 2 O 2 at a final concentration of 0.5%, 1%, 1.5% and 2%. The incubated enzyme (without inhibitors, detergents ...

After fixed in 4% PFA and repeated washing in glicine-PBS solution (100 mM, 15 min, 22°C), cells were permeabilized with Triton-X-100 (0.5 v/v%, 10 min ... Samples were subjected to SDS-PAGE (10% gels ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. This study identifies a pathway for oxidation ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Impaired insulin receptor signaling is ...

Correspondence to Professor Richard J Traub, Department of Neural and Pain Sciences, University of Maryland School of Dentistry, 650 West Baltimore Street, Baltimore, MD 21201, USA; ...

Cells cultured on coverslips were fixed with 4% paraformaldehyde for 10 min at room temperature, permeabilized with 0.2% Triton-X-100 and blocked with 0.1 M glycine, 1% BSA and 0.1% Tween20 in PBS for ...

Vivo X100 mobile was launched on 13th November 2023. The phone comes with a 120 Hz refresh rate 6.78-inch touchscreen display offering a resolution of 1260x2800 pixels and an aspect ratio of 20:9.