This is a useful study that adds new data to how different DAG pools influence cellular signaling, and dissects how the enzyme Dip2 modulates the minor lipid signaling DAG pool, which is distinct from ...

As for hardware, we established a data server with petabyte storage, thereby enabling efficient data sharing ... min at room temperature (RT ∼23 °C), permeabilized with 0.2% Triton X-100 (Fujifilm ...

Tissue was processed in ice cold buffer containing 1M Tris-Cl, 6M NaCl, 0.5M EDTA, and Triton-X-100. Protein concentrations were determined using a BCA protein assay (Thermo Fisher, Waltham, MA).

The senescence-associated markers p16, p21, p53 and beta galactosidase were induced upon drug treatment, and this was associated with increased lipid storage, increased expression of lipid ...

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School of Chemical and Process Engineering, University of Leeds, Leeds, LS2 9JT, United Kingdom ...