For maximum lysis, 20% Triton X-100 solution was added. After three hours of cocultivation, 20 µl of supernatant was transferred to another 96-well flat-bottom plate, and 200 µl of europium solution ...

And then, the slices were washed three times in 1 x PBS for 10 min each, followed by permeabilization in 1% triton X-100 solution in 1 x PBS for 40 min at room temperature. After blocking for 1 hour ...

The sections were then rinsed with PBS-Triton X-100 solution and incubated for 1 hour with the secondary antibody (1:100; rabbit anti-sheep IgG antibody (H + L), fluorescein, cat. no. FI-6000; Vector ...