0.5% SDS] and 0.5 volume of triton dilution buffer [100mM Tris-HCl pH 8.6, 100mM NaCl, 5mM EDTA pH 8, 5% Triton X-100]) supplemented with protease/phosphatase inhibitor cocktail, and samples were ...

Triton X-100, SDS at a final concentration of 0.25%, 0.5% and 1%; and an oxidizing agent-H 2 O 2 at a final concentration of 0.5%, 1%, 1.5% and 2%. The incubated enzyme (without inhibitors, detergents ...

In re-ChIP experiments, the first ChIP (H2A.Z or H2A) was eluted with 10 mM Tris-EDTA and 20 mM DTT and diluted 20 times in dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl ...

Protein samples were separated on a 12% SDS-polyacrylamide gel electrophoresis (PAGE ... Platelets and U251-MG cells were permeabilized using 0.1% Triton X-100 in PBS for 10 min at RT. Coverslips were ...