This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to ...
This important study reveals a new mechanism for gene regulation in neurons by an RNA binding protein called RBM20 previously studied in the heart. The methods used are compelling, including the ...
After separation on a 10% SDS-PAGE gel, the proteins were transferred to PVDF ... frozen sections of aortic valves were fixed in 4% PFA for 30 min and then permeabilized with 0.1% Triton X-100 in PBS ...
The sections were incubated with blocking solution (10% normal goat serum (Vector Laboratories) and 0.3% Triton X-100 (Wako) in phosphate buffer) for 2 h at room temperature. After blocking, they were ...
Protein extracts (50 μg for AGS and HCT116 and 70 μg for MKN45) were analysed by standard SDS-PAGE, transferred to a nitrocellulose membrane (Amersham Biosciences, Buckinghamshire, UK) and blotted ...
Cracow University of Technology, CUT Doctoral School, Faculty of Chemical Engineering and Technology, Warszawska 24, Cracow 31-155, Poland ...
School of Chemical and Process Engineering, University of Leeds, Leeds, LS2 9JT, United Kingdom ...
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