Sigma), Propidium iodide (PI), N-phenyl naphthylamine (NPN), Triton X-100 and Carboxy-methylcellulose (CMC) were procured from Sigma. Cell culture reagents 1X DMEM, 1X DPBS, Penicillin-Streptomycin ...
After blocking in 5% NDS, 0.5% Triton X-100 in TBS at 4°C overnight, samples were incubated at 4°C overnight with the primary NICD antibody diluted in blocking buffer (1:400). After washes, embryos ...