This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to ...

The sections were incubated with blocking solution (10% normal goat serum (Vector Laboratories) and 0.3% Triton X-100 (Wako) in phosphate buffer) for 2 h at room temperature. After blocking, they were ...

The brains and cibaria of 3-10-day-old female flies were dissected in PBST dissection buffer containing 0.015% Triton X-100 in 1x PBS, followed by fixation in 4% PFA solution for 30 minutes on a ...

Then, cells were fixed with 4% PFA for 10 min at RT and blocked/permeabilized for 20 min at RT (5% donkey serum with 0.3% Triton X-100 in PBS). After blocking, samples were stained with primary ...