High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL CA-360), 1% DOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8 ...
Binding buffer: 5 mM Tris-HCl (pH 7.5), 0.5 mM EDTA, 1 M NaCl Breaking buffer: 10 mM Tris-HCl (pH 7.5), 1% Triton-X 100, 1% SDS, 100 mM NaCl, 1 mM EDTA ...
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